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Chromatin Immunoprecipitation
English | 2024 | ISBN: 1071640704 | 288 Pages | PDF (True) | 13 MB
This comprehensive guide delves into the protocols and strategies for investigating gene regulation both experimentally and computationally. It focuses on the use of Chromatin Immunoprecipitation (ChIP) coupled with next-generation sequencing, providing a robust framework for profiling DNA-binding proteins, examining DNA footprinting, and analyzing histone modifications. As part of the highly successful Methods in Molecular Biology series, this book offers introductions on the topics covered, detailed lists of the necessary materials and reagents, step-by-step laboratory protocols that are easy to follow, and expert tips for troubleshooting and avoiding known pitfalls.

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