Biophysical Analysis of Membrane Proteins: Investigating Structure and Function By
2007 | 368 Pages | ISBN: 3527316779 | PDF | 5 MB
Meeting the need for a book on developing and using new methods to investigate membrane proteins, this is the first of its kind to present the full range of novel techniques in one resource. Top researchers from around the world focus on the physical principles exploited in the different techniques, and provide examples of how these can bring about important new insights. Following an introduction, further sections discuss structural approaches, molecular interaction and large assemblies, dynamics and spectroscopies, finishing off with an exploration of structure-function relationships in whole cells.Content: Chapter 1 High?Resolution Structures of Membrane Proteins: From X?Ray Crystallography to an Integrated Approach of Membranes (pages 1-27): Prof. Eva Pebay?PeyroulaChapter 2 Membrane Protein Structure Determination by Electron Cryo?Microscopy (pages 29-54): Christopher G. Tate and John L. RubinsteinChapter 3 Introduction to Solid?State NMR and its Application to Membrane Protein-Ligand Binding Studies (pages 55-87): Krisztina Varga and Anthony WattsChapter 4 Analytical Ultracentrifugation: Membrane Protein Assemblies in the Presence of Detergent (pages 89-120): Christine Ebel, Jesper V. Moller and Marc le MaireChapter 5 Probing Membrane Protein Interactions with Real?Time Biosensor Technology (pages 121-140): Iva Navratilova, David G. Myszka and Rebecca L. RichChapter 6 Atomic Force Microscopy: High?Resolution Imaging of Structure and Assembly of Membrane Proteins (pages 141-158): Simon Scheuring, Nikolay Buzhynskyy, Rui Pedro Goncalves and Szymon JaroslawskiChapter 7 Molecular Dynamics Studies of Membrane Proteins: Outer Membrane Proteins and Transporters (pages 159-185): Syma Khalid, John Holyoake and Mark S. P. SansomChapter 8 Understanding Structure and Function of Membrane Proteins Using Free Energy Calculations (pages 187-211): Christophe Chipot and Klaus SchultenChapter 9 Neutrons to Study the Structure and Dynamics of Membrane Proteins (pages 213-240): Kathleen Wood and Giuseppe ZaccaiChapter 10 Circular Dichroism: Folding and Conformational Changes of Membrane Proteins (pages 241-258): Nadege Jamin and Jean?Jacques LacapereChapter 11 Membrane Protein Structure and Conformational Change Probed using Fourier Transform Infrared Spectroscopy (pages 259-288): John E. Baenziger and Corrie J. B. daCostaChapter 12 Resonance Raman Spectroscopy of a Light?Harvesting Protein (pages 289-307): Andrew Aaron Pascal and Bruno RobertChapter 13 Energy Transfer Technologies to Monitor the Dynamics and Signaling Properties of G?Protein?Coupled Receptors in Living Cells (pages 309-334): Jean?Philippe Pin, Mohammed?Akli Ayoub, Damien Maurel, Julie Perroy and Eric Trinquet
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