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Unnatural Amino Acids Methods and Protocols | 6.03 MB
Title: Combined PDF
Author: Yu-Hsuan Tsai, Simon J. Elsässer
Category: Nonfiction, Science & Nature, Science, Chemistry, Organic, Biological Sciences, Biochemistry
Language: English | 424 Pages | ISBN: 1617793302
Description:
This detailed volume explores non-canonical amino acids (ncAAs) through their site-specific incorporation by genetic code expansion (GCE). The collection provides a broad resource of methods for implementing GCE in E. coli, mammalian cells, and animals, highlighting specific applications ranging from fluorescence labeling to photocontrol and the study of protein post-translational modification. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Genetically Incorporated Non-Canonical Amino Acids: Methods and Protocols serves as an ideal source of methodologies that can be adapted and extended, migrated to different model systems, and combined in new ways to help explore a wide range of biological questions and to augment industrial and pharmaceutical protein engineering.
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